Influence of aluminum hydroxide adjuvant on a murine model of allergic rhinitis

ObjectiveTo investigate the influence of aluminum hydroxide adjuvant on a murine model of allergic rhinitis(AR)and to confirm an appropriate method of establishing a mouse model of AR.Method:Establishing two types of BALB/c mice models of AR,one was identified as Local group which was characterized through intranasal sensitization and challenge using ovalbumin(OVA),and the other Systemic group which was made by intraperitoneal sensitization with OVA plus aluminum hydroxide and intranasal challenge through OVA.Then the numbers of sneezing and nasal rubbing were counted after the last challenge and the eosinophils in the nasal mucosa of mice models were observed and counted though Luna stain.Furthermore,morphological hyperplasia was examined in intraepithelial goblet cells and submucosal glands with HE stain.In addition,interlukin(IL)-4,IL-5, OVA specific IgE(sIgE)and interferon(IFN)-γin nasal lavage fluid(NLF)and serum of mice were examined using enzyme linked immunosorbent assay(ELISA).Result:The counts of sneezing and nasal rubbing in local group were more than those in systemic group and eosinophilia in the nasal mucosa of former group was greater than that in the latter one.Morphological hyperplasia was stronger in intraepithelial goblet cells and submucosal glands in local group compared with that in systemic group.Furthermore,the contents of IL-4,IL-5and sIgE increased in the NLF and serum of mice of local group compared to those of systemic one.However,the production of IFN-γ of mice in local group decreased when compared with that in Systemic group.Conclusion:OVA plus aluminum hydroxide adjuvant may promote Th1type immune response as well as Th2response.OVA intranasal sensitization and challenge locally is an appropriate way in the establishment of AR mice models.