生物传感器
登革热
登革热病毒
等离子体子
DNA
寡核苷酸
微流控
材料科学
病毒
注意事项
生物标志物
纳米技术
病毒学
计算生物学
光电子学
生物
医学
生物化学
护理部
作者
Abraham Vázquez-Guardado,Freya Mehta,Beatriz Jiménez,Aritra Biswas,Keval Ray,Aliyah Baksh,Sang Lee,Nileshi Saraf,Sudipta Seal,Debashis Chanda
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-09-08
卷期号:21 (18): 7505-7511
被引量:23
标识
DOI:10.1021/acs.nanolett.1c01609
摘要
The rapid spread of viral infections demands early detection strategies to minimize proliferation of the disease. Here, we demonstrate a plasmonic biosensor to detect Dengue virus, which was chosen as a model, via its nonstructural protein NS1 biomarker. The sensor is functionalized with a synthetic single-stranded DNA oligonucleotide and provides high affinity toward NS1 protein present in the virus genome. We demonstrate the detection of NS1 protein at a concentration of 0.1–10 μg/mL in bovine blood using an on-chip microfluidic plasma separator integrated with the plasmonic sensor which covers the clinical threshold of 0.6 μg/mL of high risk of developing Dengue hemorrhagic fever. The conceptual and practical demonstration shows the translation feasibility of these microfluidic optical biosensors for early detection of a wide range of viral infections, providing a rapid clinical diagnosis of infectious diseases directly from minimally processed biological samples at point of care locations.
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