化学
生物物理学
蛋白质组学
生物素
纳米技术
生物系统
红灯
氯
计算生物学
生物化学
植物
生物
基因
材料科学
卟啉
作者
Benito F. Buksh,Steve D. Knutson,James V. Oakley,Noah B. Bissonnette,Daniel G. Oblinsky,Michael P. Schwoerer,Ciaran P. Seath,Jacob B. Geri,Frances P. Rodriguez‐Rivera,Dann L. Parker,Gregory D. Scholes,Alexander Ploß,David W. C. MacMillan
摘要
Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited SnIV chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling in vitro through several layers of tissue, and we then apply our platform in cellulo to label EGFR microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models.
科研通智能强力驱动
Strongly Powered by AbleSci AI