Sensitive Metal Oxide-Clay Nanocomposite Colorimetric Sensor Development for Aflatoxin Detection in Foods: Corn and Almond

The work reports on zinc oxide bentonite nanocomposite (ZnOBt) chemical route synthesis, characterization, and investigation of curcumin (Cur) functionalization for a label-free colorimetric detection of total aflatoxins (AFs) in foods. XRD of ZnO nanoparticles (NPs) confirmed the wurtzite structure (2θ = 36.2°) and that of ZnOBt showed the intercalated interlayer composite phase. The Debye–Scherrer relation calculated the crystallite size as 20 nm (ZnO) and 24.4 nm (ZnOBt). Surface morphology by SEM exhibited flower-like hexagonal, rod-shaped ZnO NPs on the bentonite surface. Colorimetric reaction involved two-stage redox reactions between ZnOBt and dye Cur followed by AFs phenolic group and Zn(Cur)OBt. Cur gets oxidized at its diketone moiety in the presence of ZnOBt to form a red colored complex Zn(Cur)OBt, which further scavenge protons from AFs phenolic group, and gets oxidized to AFs-Zn(Cur)OBt (yellow). Binding of AFs-Zn(Cur)OBt is characterized by FT-IR ascribed to C–H bending (1966.615 cm–1), O–H stretching (3256.974 cm–1), and C═O stretching (1647.362 cm–1). 1H NMR chemical shifts (δ) (ppm) showed an increase in proton at the aliphatic region (0 to 4.4) while removal of proton in ether at 4.4 to 6 regions. Job plot calculation using UV–Vis data resulted in a higher total AF binding coefficient of Zn(Cur)OBt (Ka = 3.77 × 106 mol–1 L) compared to Zn(Cur)O (Ka = 0.645 × 106 mol–1 L) as well as a molar ratio of 1:1 by the Benesi–Hildebrand plot equation. Corn and almond food samples showed the total AFs LOD of 2.74 and 4.34 ppb, respectively. The results are validated with standard LC/MS-MS in compliance with MRL value as per the regulatory standard (EU).The NP-based method is facile and rapid and hence can be utilized for onsite detection of total AFs in foods.