化学
荧光团
荧光
半胱氨酸
猝灭(荧光)
谷胱甘肽
生物物理学
生物化学
量子力学
生物
物理
酶
作者
Wei Wang,Min Ji,Junqing Chen,Peng Wang
出处
期刊:Talanta
[Elsevier]
日期:2022-03-01
卷期号:239: 123091-123091
被引量:20
标识
DOI:10.1016/j.talanta.2021.123091
摘要
Biothiols, associated with multiple physiological and pathological processes, have structural similarities. Monitoring Biothiols selectively in organisms is of great significance. Burdened by the aggregation-caused quenching (ACQ) effect, the applications of conventional biothiols fluorescent probes are extremely limited. Herein, we developed a "turn-on" type aggregation-induced emission (AIE) fluorescent probe BQM-NBD, which was composed of a BQM-OH fluorophore molecule with AIE effect and the recognition group 7-nitro-1,2,3-benzoxadiazole (NBD). Non-fluorescent BQM-NBD produces strong fluorescence after the addition of cysteine (Cys) or homocysteine (Hcy). BQM-NBD exhibited excellent linearity for selective detection of Cys (0-100 Μm) and Hcy (0-50 μM) with detection limits of 6.0 × 10-8 M and 8.4 × 10-8 M, respectively. Simultaneously, after treatment with glutathione (GSH), it appeared no fluorescence. The results demonstrated BQM-NBD exhibited good selectivity to Cys/Hcy. Furthermore, BQM-NBD was successfully performed in the imaging of Cys in living cells with low cytotoxicity, which provides a feasible strategy for the selective detection of Cys in the living system.
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