安普克
脂质代谢
非酒精性脂肪肝
内分泌学
内科学
脂滴
脂肪肝
脂肪变性
自噬
胰岛素抵抗
脂肪生成
医学
化学
生物
糖尿病
磷酸化
蛋白激酶A
生物化学
疾病
细胞凋亡
作者
Hui Li,Yaoshan Dun,Wenliang Zhang,Baiyang You,Yuan Liu,Siqian Fu,Ling Qiu,Jing Cheng,Jeffrey W. Ripley-Gonzalez,Suixin Liu
出处
期刊:Life Sciences
[Elsevier]
日期:2021-05-01
卷期号:273: 119314-119314
被引量:29
标识
DOI:10.1016/j.lfs.2021.119314
摘要
To emphasize the mechanism of the effect of exercise on lipid droplet (LD) metabolism disorder in nonalcoholic fatty liver disease (NAFLD). C57BL/6J mice were randomly divided into three groups: The first group was fed with a normal diet (CON), the second group was fed a high-fat diet (HF), and finally group with a high-fat diet intervention and swim training (HF-EX). The total intervention period was 16 weeks. RT-PCR and Western blot were performed to evaluate the effect of exercise on LDs metabolism and the AMPK pathway. Histopathological examinations and immunofluorescence were performed to evaluate the lipid deposition and lipophagy in the liver. Exercise reduced liver steatosis and insulin resistance along with the stimulation of AMPK/SIRT1 signaling and downstream regulation of lipid metabolism. In addition, exercise increased the expression of autophagy marker and colocalization of LC3 and LAMP1 with LDs. Exercise stimulated AMPK/SIRT1 and activated lipophagy in NAFLD. Enhancing lipophagy may be one of the key mechanisms of regulation and resolution of NAFLD by exercise.
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