赖氨酸
酰胺酶
金黄色葡萄球菌
微生物学
生物膜
噬菌体
肌病毒科
肽聚糖
生物
溶解循环
细菌
自溶素
化学
噬菌体疗法
细菌细胞结构
葡萄球菌
病毒学
大肠杆菌
溶葡萄球菌酶
抗菌剂
病毒
生物化学
基因
遗传学
作者
Houqi Ning,Hong Lin,Jingxue Wang,Xu He,Xiaoqian Lv,Lei Ju
标识
DOI:10.1016/j.enzmictec.2021.109809
摘要
Staphylococcus aureus (S. aureus), particularly methicillin-resistant S. aureus (MRSA), and its biofilms are great threats in the food industry. Bacteriophage-encoded endolysins are promising tools to inhibit pathogens and to eliminate their biofilms. In this work, a virulent phage qdsa002 against S. aureus ATCC43300 (MRSA) was isolated, and the phage's endolysin (Lys84) and its domains were expressed and purified. Morphological and genome analyses demonstrated that qdsa002 is a Twort-like phage from Myoviridae. Lys84 contains two catalytic domains (CHAP and Amidase_2) and one cell binding domain (SH3b). This endolysin exhibits a strong lytic activity against S. aureus and has a wider bactericidal spectrum than qdsa002. Moreover, Lys84 exceed 10 μM effectively removed around 90 % of the biofilms of S. aureus. Besides, CHAP and Amidase_2 domains remained 61.20 % and 59.46 % of lytic activity as well as 84.31 % and 70.11 % of anti-biofilm activity of Lys84, respectively. The lytic and anti-biofilm activities of the combination of CHAP and Amidase_2 were close to 90 % of those of Lys84. These results indicated that Lys84 and its domains might be alternative antimicrobials for controlling S. aureus and its biofilms.
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