京尼平
明胶
化学
间充质干细胞
组织工程
粘附
生物活性
细胞粘附
血管内皮生长因子
生物物理学
生物医学工程
血管内皮生长因子受体
细胞
生物化学
细胞生物学
壳聚糖
体外
生物
有机化学
医学
癌症研究
作者
Pello Sánchez,José Luís Pedraz,Gorka Orive
标识
DOI:10.1016/j.ijbiomac.2016.12.092
摘要
We have designed, developed and optimized Genipin cross-linked 3D gelatin scaffolds that were biologically active and biomimetic, show a dual activity both for growth factor and cell delivery. Type B gelatin powder was dissolved in DI water. 100 mg of genipin was dissolved in 10 ml of DI water. Three genipin concentrations were prepared: 0.1%, 0.2% and 0.3% (w/v). Solutions were mixed at 40 °C and under stirring and then left crosslinking for 72 h. Scaffolds were obtained by punching 8 mm-cylinders into ethanol 70% solution for 10 min and then freeze-drying. Scaffolds were biologically, biomechanically and morphologically evaluated. Cell adhesion and morphology of D1-Mesenchymal stem cells (MSCs) and L-929 fibroblast was studied. Vascular endothelial grwoth factor (VEGF) and Sonic hedgehog (SHH) were used as model proteins. Swelling ratio increased and younǵs module decreased along with the concentration of genipin. All scaffolds were biocompatible according to the toxicity test. MSC and L-929 cell adhesion improved in 0.2% of genipin, obtaining better results with MSCs. VEGF and SHH were released from the gels. This preliminary study suggest that the biologically active and dual gelatin scaffolds may be used for tissue engineering approaches like bone regeneration.
科研通智能强力驱动
Strongly Powered by AbleSci AI