单细胞蛋白
生物量(生态学)
Plackett–伯曼设计
食品科学
化学
响应面法
异养
细菌
实验室烧瓶
发酵
制浆造纸工业
生物
色谱法
生态学
遗传学
物理化学
工程类
作者
Jemila J. Nunes,Brian Aufderheide,Devatie M. Ramjattan,Renuka Dass
出处
期刊:The Journal of Microbiology, Biotechnology and Food Sciences
[Slovak University of Agriculture in Nitra]
日期:2016-12-01
卷期号:6 (3): 894-899
被引量:11
标识
DOI:10.15414/jmbfs.2016/17.6.3.894-899
摘要
The growing global demand for nutritional protein means that a sustainable source such as Single Cell Protein from Methylococcus capsulatus (Bath) can become a potential replacement for fishmeal and other animal feeds. Improving biomass concentrations using statistical optimization during synergistic fermentations with a mixed consortium of the three heterotrophic bacteria Alcaligenes acidovorans, Aneurinibacillus danicus, and Brevibacillus sp. can increase the feasibility of the industrial process. The medium components Mg2+, Ca2+, Fe3+, Cu2+, PO42-, NO3-, MoO42-, trace metals, and process temperature were screened using a two-level Plackett-Burman Design in shake flasks which resulted in Cu2+ being the only significant factor. The optimum level of CuSO4.5H2O was found to be 40 μM using One Factor Response Surface Methodology, which was three times higher than the typical values of Cu2+ used previously. These combined strategies led to a 265% increase in biomass, with final cell concentration of 10.3 g/L, up from 2.8 g/L in fed batch fermentations over 48 hours. The heterotrophic bacteria did not grow on NMS or methane but increased biomass concentration when added to M. capsulatus (Bath) cultures.
科研通智能强力驱动
Strongly Powered by AbleSci AI