The essential oils isolated from the aerial parts of two different populations of Artemisia gmelinii growing in Uttarakhand Himalaya region were analysed by gas chromatography and gas chromatography/mass spectrometry (GC-MS) in order to determine the variation of concentration in their constituents. Artemisia ketone was detected as a major constituent in both the populations i.e., Niti valley and Jhelum samples. Niti oil was found to have considerably greater amounts of artemesia ketone (53.34%) followed by α-thujone (9.91%) and 1,8-cineole (6.57%), Similarly, the first major compound in Jhelum oil was artemesia ketone (40.87%), whereas ar-curcumene (8.54%) was identified as a second major compound followed by α-thujone (4.04%). Artemisia ketone can be useful for perfumery and fragrance to introduce new and interesting herbaceous notes.