Simultaneous Determination of 15 Flavonoids in Scutellaria barbata–Hedyotis diffusa Herb Pair by HPLC Q-TOF MS

色谱法 黄芩 化学 黄芩苷 草本植物 芦丁 高效液相色谱法 芹菜素 槲皮素 传统医学 类黄酮 医学 草药 中医药 抗氧化剂 替代医学 病理 生物化学
作者
Wei Yang,Shiyong He,Nan Xiao,Yujie Qiao,Hua Sui,Lina Liang,Jing Chen,Weiling Li,Lin Zhang
出处
期刊:Journal of AOAC International [Oxford University Press]
卷期号:102 (1): 75-80 被引量:12
标识
DOI:10.5740/jaoacint.17-0469
摘要

Background: The Scutellaria barbata-Hedyotis diffusa herb pair has appeared in classical prescriptions and has usually been used for the treatment of cancer and inflammatory conditions. Objective: For quality control of this herb pair, a rapid and reliable HPLC-quadrupole-time-of-flight(Q-TOF)-MS method was used for the simultaneous determination of alpinetin, apigenin-7-O-β-D-glucopyranoside, scutellarein, apigenin, quercetin-3-O-β-D-glucopyranoside, wogonoside, quercetin, amentoflavone, wogonin, chrysin, luteolin, rutin, naringenin, baicalein, and baicalin in the S. barbata-H. diffusa herb pair. Methods: LC-MS analysis was performed on a Shimadzu HPLC 20ADXR LC system in-line with an AB-SCIEX 5600 Triple TOF mass spectrometer. Results: The calibration curves showed good linearity in a relatively wide concentration range (r >0.996). The LOD and LOQ were less than 20 and 68 ng/mL, respectively. The relative standard deviation (RSD) values of intraday and inter-day precision were <3.31% for all the analytes. The recoveries of the method were in the range of 94.6-106.0%, with RSD <4.72%. The RSD values of peak areas in the stability study were not more than 4.28% for all analytes within 2 days. These results revealed that the method exhibited good sensitivity, precision, accuracy, and stability. Conclusions: This method could be applied to the quantitative determination of 15 flavonoids in the S. barbata - H. diffusa herb pair. Highlights: (1) The established method of HPLC-Q-TOF-MS is simple, fast, sensitive, and reliable. (2) 15 Flavonoids in the S. barbata-H. diffusa herb pair were simultaneous determined.
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