Community structure and classification of Actinobacteria in parallel AO/AN technology for phosphorus removal

Actinobacteria community structure was studied in a parallel AO/NO phosphorus removal process under recirculating conditions,and the microorganism′s structure and behavior were analyzed.The community structure of Actinobacteria was also determined by directly obtaining DNA from samples of activated sludge,followed by nested PCR and DGGE.The community structure of Actinobacteria was analyzed by sequencing 16 rDNA V3 area gene fragments from the activated sludge.By comparing the gene sequences with those in the NCBI(National Center of Biological Information),the microorganisms present were determined.Analyzing the small changes of preponderant bacteria in anaerobic/aerobic and anaerobic/anoxic conditions takes know,that under the stable phosphorus removal conditions,the system can be kept mostly constant.The type and amount of the microorganisms is correlated with the oxygen level in the system,but the Actinobacteria structure can adapt to the environmental conditions of the processes.