Study on immobilization of Pseudomonas strain degradation of formaldehyde

A formaldehyde-degrading bacterium strain was screened from the activated sludges,which was named W1.The strain was preliminarily identified as Pseudomonas according to its morphology and biochemical test.Meanwhile,the growth and degradation characteristics of strains W1 were preliminarily studied with entrapped in sodium alginate as the carrier.W1 was immobilized with sodium alginate of different concentrations and added with different bacterial suspension.The optimum conditions of formaldehyde degradation efficiency by L9(33)orthogonal experimental design were as follows:concentration of(NH4)2SO42.4g/L,MgSO4·7H2O 0.2g/L,mother liquors of trace elements of 0.1mL,pH value of9.0,incubation temperature of 30℃.The results showed that the removal efficiency of formaldehyde of strain W1 could be as high as 80.3%in 48 hunder optimal conditions.The experiment established the basis for the application of biological removal of formaldehyde based on the technique of immobilized microorganism.