A Novel Method for Estimating Chlorophyll and Carotenoid Concentrations in Leaves: A Two Hyperspectral Sensor Approach

Leaf optical properties can be used to identify environmental conditions, the effect of light intensities, plant hormone levels, pigment concentrations, and cellular structures. However, the reflectance factors can affect the accuracy of predictions for chlorophyll and carotenoid concentrations. In this study, we tested the hypothesis that technology using two hyperspectral sensors for both reflectance and absorbance data would result in more accurate predictions of absorbance spectra. Our findings indicated that the green/yellow regions (500-600 nm) had a greater impact on photosynthetic pigment predictions, while the blue (440-485 nm) and red (626-700 nm) regions had a minor impact. Strong correlations were found between absorbance (R