内分泌学
内科学
甘氨酸受体
链脲佐菌素
糖尿病神经病变
脊髓
糖尿病
医学
受体
神经病理性疼痛
甘氨酸
周围神经病变
化学
药理学
生物化学
氨基酸
精神科
作者
Miguel Ángel Velázquez-Flores,Gustavo Sánchez‐Chávez,Sara L. Morales‐Lázaro,Ruth Ruiz Esparza‐Garrido,Alejandro Canizales-Ontiveros,Rocı́o Salceda
标识
DOI:10.1007/s11064-023-04058-9
摘要
In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1-α3 and β) in streptozotocin-induced diabetic Long-Evans rats by qPCR and Western blot. The total mRNA and protein expression (whole spinal cord homogenate) of the α1, α3, and β subunits did not change during diabetes; however, the α2 subunit mRNA, but not the protein, was overexpressed 45 days after diabetes induction. By contrast, the synaptic expression of the α1 and α2 subunits decreased in all the studied stages of diabetes, but that of the α3 subunit increased on day 45 after diabetes induction. Intradermal capsaicin produced higher paw-licking behavior in the streptozotocin-induced diabetic rats than in the control animals. In addition, the nocifensive response was higher at 45 days than at 20 days. During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy.
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