费斯特共振能量转移
荧光
化学
条形码
DNA
多路复用
生物分析
纳米结构
解码方法
计算生物学
分析化学(期刊)
纳米技术
材料科学
生物
计算机科学
物理
色谱法
生物化学
光学
算法
电信
操作系统
作者
Xiaoshuang Zhao,Yi Xu,Ziting Chen,Chenxiao Tang,Xianqiang Mi
标识
DOI:10.1016/j.bios.2023.115994
摘要
Biocoding technology constructed by readable tags with distinct signatures is a brand-new bioanalysis method to realize multiplexed identification and bio-information decoding. In this study, a novel fluorescence intensity coding technology termed Tetra-FICT was reported based on tetrahedron DNA nanostructure (TDN) carrier and Főrster Resonance Energy Transfer (FRET) effect. By modulating numbers and distances of Cy3 and Cy5 at four vertexes of TDN, different fluorescence intensities of twenty-six samples were produced at ∼565.0 nm (FICy3) and ∼665.0 nm (FICy5) by detecting fluorescence spectra. By developing an error correction mechanism, eleven codes were established based on divided intensity ranges of the final FICy3 together with FICy5 (Final-FICy3&FICy5). These resulting codes were used to construct barcode probes, with three miRNA biomarkers (miRNA-210, miRNA-199a and miRNA-21) as cases for multiplexed bio-assay. The high specificity and sensitivity were also demonstrated for the detection of miRNA-210. Overall, the proposed Tetra-FICT enriched the toolbox of fluorescence coding, which could be applied to multiplexing biomarkers detection.
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