细胞仪
材料科学
表征(材料科学)
流式细胞术
电阻抗
生物医学工程
纳米技术
生物系统
生物物理学
物理
生物
医学
遗传学
量子力学
作者
Yongxiang Feng,Jing Zhu,Huichao Chai,Weihua He,Liang Huang,Wenhui Wang
出处
期刊:Small
[Wiley]
日期:2023-07-12
标识
DOI:10.1002/smll.202303416
摘要
Reflecting various physiological states and phenotypes of single cells, intrinsic biophysical characteristics (e.g., mechanical and electrical properties) are reliable and important, label-free biomarkers for characterizing single cells. However, single-modal mechanical or electrical properties alone are not specific enough to characterize single cells accurately, and it has been long and challenging to couple the conventionally image-based mechanical characterization and impedance-based electrical characterization. In this work, the spatial-temporal characteristics of impedance sensing signal are leveraged, and an impedance-based multimodal electrical-mechanical flow cytometry framework for on-the-fly high-dimensional intrinsic measurement is proposed, that is, Young's modulus E, fluidity β, radius r, cytoplasm conductivity σi , and specific membrane capacitance Csm , of single cells. With multimodal high-dimensional characterization, the electrical-mechanical flow cytometry can better reveal the difference in cell types, demonstrated by the experimental results with three types of cancer cells (HepG2, MCF-7, and MDA-MB-468) with 93.4% classification accuracy and pharmacological perturbations of the cytoskeleton (fixed and Cytochalasin B treated cells) with 95.1% classification accuracy. It is envisioned that multimodal electrical-mechanical flow cytometry provides a new perspective for accurate label-free single-cell intrinsic characterization.
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