透明质酸
阿霉素
聚合物囊泡
CD44细胞
化学
内化
细胞毒性
药物输送
体内分布
体内
癌症研究
生长抑制
生物物理学
细胞凋亡
体外
细胞
生物化学
共聚物
化疗
医学
生物
两亲性
内科学
聚合物
有机化学
生物技术
解剖
作者
Morvarid Borhaninia,Mahsa Zahiri,Khalil Abnous,Seyed Mohammad Taghdisi,Mohammad Ramezani,Mona Alibolandi
标识
DOI:10.1016/j.ijbiomac.2023.125882
摘要
In this study, a targeted pH-sensitive polymersome incorporating doxorubicin (DOX) was manufactured implementing diblock copolymer of hyaluronic acid-b-pPoly (β-amino ester) (HA-PBAE). The hydrophilic DOX was loaded into the aqueous compartment of HA-PBAE polymersomal structure during nanoprecipitation process with 60 % ± 3.0 entrapment efficiency (EE%) and 5.3 % ± 0.2 loading content (LC%) while demonstrating spherical morphology with size of 196 ± 3.8 nm and PDI of 0.3. The prepared platform (DOX-HA-PBAE) illustrated accelerated DOX release in acidic pH 5.4, and showed significantly higher cytotoxicity and cellular internalization in comparison with free DOX against 4T1 cell line (CD44 positive cell). In contrast, no significant growth inhibition was observed in CHO cell line (CD44 negative cell). Furthermore, DOX-HA-PBAE platform displayed higher therapeutic efficacy, favorable tumor accumulation and lower systemic toxicity in comparison with free DOX based on obtained experimental data in ectopic 4T1 tumor model in BALB/c Female mice in terms of tumor growth rate, survival rate, body weight loss, ex vivo biodistribution and pathological evaluations. The obtained results demonstrated that DOX-HA-PBAE polymersomes have potential to be used in metastatic breast cancer therapy with promising characteristics in terms of tumor growth suppression and safety profile.
科研通智能强力驱动
Strongly Powered by AbleSci AI