促炎细胞因子
纳米载体
巨噬细胞极化
细胞生物学
肿瘤坏死因子α
活性氧
材料科学
化学
巨噬细胞
生物
炎症
纳米技术
体外
生物化学
免疫学
药物输送
作者
Jialiang Li,Fan Jia,Yan Gao,Shuodan Huang,Di Huang,Jiachen Li,Xiaoyu Wang,Hélder A. Santos,Pingping Shen,Bing Xia
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-01-04
卷期号:17 (2): 1036-1053
被引量:6
标识
DOI:10.1021/acsnano.2c07439
摘要
The development of nanosystems with intrinsic immunomodulatory effects on macrophage polarization is important for the macrophage-targeted immunotherapy. Here, mitochondria-targeted bovine serum albumins (BSAs) via the conjugation of fluorescent, lipophilic, and cationic rhodamine 110 molecules can efficiently enhance the gene expression of the proinflammatory phenotype of macrophages and correspondingly inhibit the gene expression of their anti-inflammatory phenotype. On this basis, porous silicon nanocarriers can further boost the immunomodulation of these mitochondria-targeted BSAs in vitro or in vivo, accompanied by the secretion of proinflammatory mediators including tumor necrosis factor α, nitric oxide, and reactive oxygen species (ROS). Meanwhile, BSA coatings can also improve the biocompatibility of porous silicon nanoparticulate cores on macrophages. Finally, the mechanism investigations demonstrate that porous silicon nanocarriers can efficiently deliver mitochondria-targeted BSA into macrophages to generate mitochondrial ROS via the interference with mitochondrial respiratory chains, which can further trigger the downstream signaling transduction pathways for the proinflammatory transition. Considering the good biosafety and versatile loading capability, this developed porous silicon@BSA nanosystem with a strong proinflmmatory regulatory effect has important potential on the combinatorial chemoimmunotherapy against cancer or viral/bacterial-related infectious diseases.
科研通智能强力驱动
Strongly Powered by AbleSci AI