纳米团簇
细菌
大肠杆菌
荧光
化学
抗菌剂
体内
金黄色葡萄球菌
生物膜
细菌细胞结构
微生物学
荧光显微镜
革兰氏阴性菌
荧光寿命成像显微镜
肽
细胞毒性
组合化学
生物物理学
生物化学
生物
体外
有机化学
遗传学
物理
生物技术
量子力学
基因
作者
Yunqiu Shen,Chaochuan Zheng,Qiaoli Wu,Qilong Wu,Ming Jin,Yayun Jiang,Fuyuan Huang,Yongliang Lou,Laibao Zheng
标识
DOI:10.3389/fmicb.2022.1003359
摘要
The development of multifunctional nanomaterials with bacterial imaging and killing activities is of great importance for the rapid diagnosis and timely treatment of bacterial infections. Herein, peptide-functionalized gold nanoclusters (CWR11-AuNCs) with high-intensity red fluorescence were successfully synthesized via a one-step method using CWR11 as a template and by optimizing the ratio of CWR11 to HAuCl4, reaction time, pH, and temperature. The CWR11-AuNCs bound to bacteria and exhibited selective fluorescence microscopy imaging properties, which is expected to provide a feasible method for locating and imaging bacteria in complex in vivo environments. In addition, CWR11-AuNCs not only retained the antibacterial and bactericidal activities of CWR11 but also exhibited certain inhibitory or killing effects on gram-negative and gram-positive bacteria and biofilms. The MICs of CWR11-AuNCs against Escherichia coli and Staphylococcus aureus were 178 and 89 μg/ml, respectively. Surprisingly, cell viability in the CWR11-AuNC-treated group was greater than that in the CWR11-treated group, and the low cytotoxicity exhibited by the CWR11-AuNCs make them more promising for clinical applications.
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