Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy

粒体自噬 生物 竞争性内源性RNA 自噬 品脱1 细胞生物学 基因敲除 核糖核酸 遗传学 长非编码RNA 基因 细胞凋亡
作者
Xuejun Wang,Qinghong Lin,Li Tian,M Kellis,Teruko Fukuyama,Weijung Ten,Xiehe Kong,Yanting Yang,Xiaopeng Ma,Xingtao Zhou
出处
期刊:Chinese Medicine [BioMed Central]
卷期号:20 (1)
标识
DOI:10.1186/s13020-025-01058-5
摘要

Abstract Background Mitophagy is closely related to the regulation of retinal ganglion cell (RGC) structure and function. Our previous study suggested that long noncoding RNAs (lncRNAs) can cause damage to myopic RGCs. However, whether electroacupuncture (EA) treatment can delay myopia progression through lncRNA-mediated mitophagy in RGCs is currently unknown. This study aimed to investigate the effect of EA on lncRNA-mediated mitophagy in myopic RGCs. Methods Our study investigated the modulatory effect of EA on mitophagy in RGCs of guinea pigs with form-deprived myopia (FDM). RNA sequencing was performed to further analyze the expression profiles of lncRNAs and mRNAs in RGCs of guinea pigs with FDM after EA treatment, and the related competing endogenous RNA (ceRNA) network was constructed. Importantly, PINK1, a mitophagy-related gene, was included in the core ceRNA network to explore the relationship between lncRNAs and mitophagy in myopic RGCs regulated by EA. We also collected eyeballs from myopic and highly myopic adults to further verify the mechanistic results. Results This study demonstrated that EA treatment delayed the reduction in refraction and increase in axial length and alleviated RGC damage in guinea pigs with FDM. We further found that EA could induce mitophagy in guinea pig RGCs with FDM by promoting the mitophagy-related PINK1/Parkin signaling pathway. Moreover, mitophagy is inhibited in the retina of highly myopic adults. RNA sequencing revealed that 599 lncRNAs and 455 mRNAs were differentially expressed in guinea pig RGCs with FDM after EA treatment. A core ceRNA network was constructed by incorporating PINK1 and verified by related molecular experiments, and we found that EA treatment may induce mitophagy and attenuated RGC injury in guinea pigs with FDM by sponging miR-342-5p through lncRNA-XR_002789763.1 to activate the PINK1/Parkin signaling pathway and promote Mfn2 ubiquitination. Conclusion EA treatment might regulate lncRNA-XR_002789763.1/miR-342-5p axis and activate the mitophagy-related PINK1/Parkin signaling pathway, and promote Mfn2 ubiquitination, thereby alleviating RGC damage and delaying myopia progression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
bioglia完成签到,获得积分10
刚刚
mj789完成签到,获得积分10
1秒前
1秒前
2秒前
zyd完成签到,获得积分10
2秒前
3秒前
3秒前
4秒前
树下发布了新的文献求助10
4秒前
打打应助Amorfati采纳,获得10
5秒前
李爱国应助mol采纳,获得10
5秒前
6秒前
8秒前
OFish发布了新的文献求助10
8秒前
ziyouren发布了新的文献求助10
9秒前
9秒前
小王发布了新的文献求助10
9秒前
10秒前
10秒前
大个应助树下采纳,获得10
10秒前
11秒前
顾矜应助苹果采纳,获得10
11秒前
小李完成签到,获得积分10
11秒前
王可爱完成签到,获得积分10
12秒前
13秒前
陶醉的向南完成签到,获得积分10
13秒前
胡萝卜完成签到,获得积分10
14秒前
15秒前
15秒前
15秒前
冲冲冲完成签到,获得积分10
16秒前
chen应助roywin采纳,获得10
17秒前
Ayo大肥猫完成签到,获得积分10
17秒前
小蘑菇应助杆杆采纳,获得10
17秒前
18秒前
笨笨醉薇发布了新的文献求助10
20秒前
20秒前
宝拉~完成签到,获得积分10
20秒前
21秒前
人各有痣完成签到,获得积分10
21秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds第二卷 1200
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038857
求助须知:如何正确求助?哪些是违规求助? 3576470
关于积分的说明 11375739
捐赠科研通 3306266
什么是DOI,文献DOI怎么找? 1819414
邀请新用户注册赠送积分活动 892755
科研通“疑难数据库(出版商)”最低求助积分说明 815066