精液
基因分型
ABO血型系统
精子
污渍
微卫星
毛细管电泳
聚合酶链反应
真空吸尘器
生物
性侵犯
打字
分子生物学
色谱法
男科
免疫学
遗传学
化学
基因型
染色
医学
等位基因
毒物控制
环境卫生
基因
伤害预防
作者
Jun Yao,Atif Adnan,Hongbo Wang
标识
DOI:10.1002/elps.202300021
摘要
In sexual assault cases, one of the most common samples collected is a mixed semen stain, which is often found on the vagina, female underwear, or bed sheets. However, it is usually difficult to identify the perpetrator based on this sample alone. One technique that has been developed to address this issue is magnetic bead-based separation. This method involves using modified magnetic microspheres to capture and enrich specific target cells, in this case, sperm cells. In this study, we utilized magnetic beads coupled with ABH blood group antibody to isolate sperm cells from an individual of a single ABO blood type. Subsequently, polymerase chain reaction amplification and capillary electrophoresis were employed to perform the genotyping the short tandem repeat (STR) loci. This approach allows for the identification of different individuals in a mixed seminal stain sample from two individuals, by first separating sperm cells based on ABH antigen differences and subsequently utilizing autosomal STR typing on the enriched single blood group cells.
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