基因敲除
血-视网膜屏障
糖尿病性视网膜病变
血管通透性
基因沉默
免疫印迹
蛋白激酶B
视网膜
PI3K/AKT/mTOR通路
医学
腹腔注射
链脲佐菌素
视网膜
化学
癌症研究
药理学
糖尿病
细胞生物学
信号转导
内分泌学
生物
眼科
细胞凋亡
生物化学
神经科学
基因
作者
Qian Yang,Chaoyang Zhang,Hai Xie,Lei Tang,Dandan Liu,Qinghua Qiu,Da Luo,Kun Liu,Jingying Xu,Haibin Tian,Lixia Lü,Guo‐Tong Xu,Jingfa Zhang
标识
DOI:10.1016/j.bbrc.2021.10.024
摘要
To examine the mechanisms of Nogo-B (RTN4B) in the protection of blood-retinal barrier in experimental diabetic retinopathy.The level of Nogo-B in vitreous and plasma samples was detected with ELISA. Diabetes was induced in Sprague-Dawley rats with intraperitoneal injection of streptozotocin. The rats were injected intravitreally with adeno-associated virus (AAV) for knockdown the expression of Nogo-B in retina or/and as AAV negative control. The permeability of blood-retinal barrier was detected with Rhodamine-B-dextran leakage assay. The expressions of Nogo-B, junctional proteins, inflammatory factors and signaling pathways were examined with Western blot and quantitative real-time PCR.Nogo-B expression was significantly upregulated in clinical samples and experimental diabetic rat models. Under normal condition, Nogo-B knockdown resulted in the increased permeability of retinal blood vessels. In diabetic rat retinas, the vascular leakage was increased significantly, which was partially decreased by Nogo-B knockdown through increasing p/t-Src (Tyr529) and p/t-Akt (Ser473), and decreasing p/t-ERK1/2.Nogo-B was increased in diabetic retinopathy and silencing Nogo-B is a promising therapy for diabetic retinopathy.
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