成牙骨质细胞
鱼腥草素骨
运行x2
骨钙素
化学
牙骨质
牙骨质
碱性磷酸酶
成骨细胞
细胞分化
细胞生物学
分子生物学
细胞生长
生物
生物化学
病理
牙本质
医学
基因
体外
酶
作者
Yunru Hao,Yunlong Wang,Mingyuan Du,Leilei Wang,Zhijian Liu,Chen Zhang,Zhengguo Cao,Hong He
标识
DOI:10.1080/00016357.2021.1966096
摘要
Cementum which is a layer of thin and bone-like mineralised tissue covering tooth root surface is deposited and mineralised by cementoblasts. Recent studies suggested long noncoding RNA H19 (H19) promotes osteoblast differentiation and matrix mineralisation, however, the effect of H19 on cementoblasts remains unknown. This study aimed to clarify the regulatory effects of H19 on cementoblast differentiation, mineralisation, and proliferation.An immortalised murine cementoblast cell line OCCM-30 was used in this study. H19 expression was examined by real-time quantitative polymerase chain reaction (RT-qPCR) during OCCM-30 cell differentiation. OCCM-30 cells were transfected with lentivirus or siRNA to up-regulate or down-regulate H19, then the levels of runt-related transcription factor 2 (Runx2), osterix (Sp7), alkaline phosphatase (Alpl), bone sialoprotein (Ibsp), osteocalcin (Bglap) were tested by RT-qPCR or western blot. Alizarin red staining, ALP activity assay and MTS assay were performed to determine the mineralisation and proliferation ability of OCCM-30 cells.H19 was dramatically increased during OCCM-30 cell differentiation. Overexpression of H19 increased the levels of Runx2, Sp7, Alpl, Ibsp, and Bglap and enhanced ALP activity and the formation of mineral nodules. While down-regulation of H19 suppressed the above cementoblast differentiation genes and inhibited ALP activity and mineral nodule formation. However, the proliferation of OCCM-30 cells was not affected.H19 promotes the differentiation and mineralisation of cementoblasts without affecting cell proliferation.
科研通智能强力驱动
Strongly Powered by AbleSci AI