高铁F1
氧化应激
热休克蛋白70
活性氧
热休克蛋白
热冲击系数
线粒体通透性转换孔
线粒体
线粒体ROS
细胞生物学
化学
生物
超氧化物
氧化磷酸化
热休克蛋白60
肾
生物化学
程序性细胞死亡
内分泌学
细胞凋亡
酶
基因
作者
Liang Yan,Namakkal S. Rajasekaran,S. Sathyanarayanan,Ivor J. Benjamin
标识
DOI:10.1089/ars.2005.7.465
摘要
Increased synthesis of heat shock proteins (Hsps), mainly regulated by heat shock factor 1 (Hsf1), protects the heart against oxidative stress under pathophysiological conditions such as ischemia/reperfusion. To investigate whether Hsps might exert a similar protective effect under physiological conditions in the kidney, we first evaluated the HSF1-dependent expression of several Hsps, including Hsp25, αB-crystallin (αBC), Hsp70, and Hsp90. Unlike either αBC or Hsp70, protein expression of Hsp25 and Hsp90 was decreased 26% and 50%, respectively, in Hsf1 knockout compared with the wild-type mice. The effects of Hsp down-regulation on renal cellular redox status are presently unknown. Indeed, HSF1 deficiency caused a 37% decrease in renal cellular GSH/GSSG ratio, a marker of redox status, and a 40% increase in the rate of mitochondrial superoxide generation in Hsf1 knockout compared with wild-type mice. HSF1 disruption also increased mitochondrial permeability transition pore opening and induced greater mitochondrial membrane potential change (48% increase versus wild type). Thus, the present study demonstrates that Hsf1-dependent transcription of selective Hsps is required for normal renal homeostasis, which protects renal cells against oxidative stress under physiological conditions. The source of mitochondrial superoxide generation is discussed.Antioxid. Redox Signal. 7, 465–471.
科研通智能强力驱动
Strongly Powered by AbleSci AI