根际
生物
温度梯度凝胶电泳
植物
大块土
稳定同位素探测
细菌
放线菌门
微生物种群生物学
微生物
16S核糖体RNA
遗传学
作者
Feth el Zahar Haichar,C. Marol,Odile Berge,J. Ignacio Rangel‐Castro,James I. Prosser,Jérôme Balesdent,Thierry Heulin,Wafa Achouak
出处
期刊:The ISME Journal
[Springer Nature]
日期:2008-08-28
卷期号:2 (12): 1221-1230
被引量:895
标识
DOI:10.1038/ismej.2008.80
摘要
Abstract The rhizosphere is active and dynamic in which newly generated carbon, derived from root exudates, and ancient carbon, in soil organic matter (SOM), are available for microbial growth. Stable isotope probing (SIP) was used to determine bacterial communities assimilating each carbon source in the rhizosphere of four plant species. Wheat, maize, rape and barrel clover (Medicago truncatula) were grown separately in the same soil under 13CO2 (99% of atom 13C) and DNA extracted from rhizosphere soil was fractionated by isopycnic centrifugation. Bacteria-assimilating root exudates were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of 13C-DNA and root DNA, whereas those assimilating SOM were identified from 12C-DNA. Plant species root exudates significantly shaped rhizosphere bacterial community structure. Bacteria related to Sphingobacteriales and Myxococcus assimilated root exudates in colonizing roots of all four plants, whwereas bacteria related to Sphingomonadales utilized both carbon sources, and were identified in light, heavy and root compartment DNA. Sphingomonadales were specific to monocotyledons, whereas bacteria related to Enterobacter and Rhizobiales colonized all compartments of all four plants, used both fresh and ancient carbon and were considered as generalists. There was also evidence for an indirect important impact of root exudates, through stimulation of SOM assimilation by a diverse bacterial community.
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