过剩4
胰岛素
染色体易位
生物
细胞生物学
化学
生物化学
内科学
医学
基因
作者
Qinghua Wang,Zayna A. Khayat,Kazuhiro Kishi,Yasuhiko Ebina,Amira Klip
出处
期刊:FEBS Letters
[Wiley]
日期:1998-05-08
卷期号:427 (2): 193-197
被引量:210
标识
DOI:10.1016/s0014-5793(98)00423-2
摘要
We report a rapid and sensitive colorimetric approach to quantitate the amount of glucose transporters exposed at the surface of intact cells, using L6 muscle cells expressing GLUT4 containing an exofacial myc epitope. Unstimulated cells exposed to the surface 5 fmol GLUT4myc per mg protein. This value increased to 10 fmol/mg protein in response to insulin as 2‐deoxyglucose (10 μM) uptake doubled. The results are substantiated by immunofluorescent detection of GLUT4myc in unpermeabilized cells and by subcellular fractionation. We further show that wortmannin and the cytoskeleton disruptors cytochalasin D and latrunculin B completely blocked these insulin effects. The rapid quantitative assay described here could be of high value to study insulin signals and to screen for potential anti‐diabetic drugs.
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