A82775b and a82775c, novel metabolites of an unknown fungus of the order sphaeropsidales

Compounds A82775B (1) and A82775C (2) were isolated as non-antimicrobial by-products during attempts to isolate an elusive G+, G- antibiotic from culture A82775. an unidentified mold. A82775 factors B and C are present in both the mycelium and filtered broth. The metabolites were recovered from the filtered broth by adsorption on Diaion HP-20 nonfunctionalized macroreticular resin and purified by preparative reverse phase HPLC on C18 bonded phase supports. The structure elucidation of A82775 factors B (1) and C (2) has been accomplished by spectroscopic means including COSY, NOESY, INAPT, HMQC and 2D-INADEQUATE NMR experiments.