真菌毒素
生物
胚胎
胚胎发生
毒性
卵母细胞
合子
呕吐毒素
男科
DNA损伤
人类受精
原核
氧化应激
细胞生物学
分子生物学
玉米赤霉烯酮
化学
遗传学
植物
DNA
生物化学
医学
有机化学
作者
Biyun Liao,Linlin Hu,Hongge Li,Yuping Xu,Shao‐Chen Sun,Junli Wang
标识
DOI:10.1093/micmic/ozad005
摘要
Deoxynivalenol is a mycotoxin, produced by Fusarium from contaminated corn, wheat, and other grains, that induces multiple effects in humans and animals, including cytotoxic, genotoxic, immunotoxic, and carcinogenic effects. Recent studies show that deoxynivalenol also affects the reproductive system of mammals, including oocyte quality. However, the effects of deoxynivalenol on early embryonic development have not been reported. In this study, fluorescence intensity analysis was used to show that deoxynivalenol disrupted the first cleavage of the zygote. The high deoxynivalenol dose disturbed the movement of the pronucleus after fertilization, while the low deoxynivalenol dose caused aberrant spindle morphology during the metaphase of the first cleavage. Further analysis showed that the reactive oxygen species level increased in the deoxynivalenol-exposed two-cell embryos, indicating oxidative stress. Moreover, deoxynivalenol caused DNA damage in the embryos, as positive γH2A.X signals were detected in the nucleus. These events led to the early apoptosis of mouse embryos, which was confirmed by autophagy. Taken together, our study provides evidence for the toxicity of deoxynivalenol during early embryonic development in the mouse model.
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