RNA-m5C regulatory atlas of human fetal tissues uncover the activities of Nsun2/Jarid2/Alyref axis

Our study presents a high-resolution, comprehensive tissue-specific m5C landscape during human fetal development, serving as a foundational resource for developmental epigenetics research. Subsequently, we identified a novel regulatory axis linking Nsun2, the Jarid2/Ezh2 complex, m5C modification patterns and Alyref functionality. This pathway integrates RNA methylation, histone modification and chromatin accessibility to precisely orchestrate fetal development, advancing our understanding of epigenetic regulation and offering new insights into potential therapeutic targets for developmental disorders.