A NIR ratiometric fluorescent probe for the rapid detection of hydrogen sulfide in living cells and zebrafish

Hydrogen sulfide (H2S) acts as a gas transporter and cell protector and plays a role in a number of disorders and signaling processes. Given that the half-life of H2S in biological systems is between seconds and minutes, the development of rapid and accurate technologies for reliable monitoring H2S levels and dynamics in organisms is critical. However, it is still difficult to design innovative near-infrared fluorescent probes that can quickly and accurately detect H2S. Here, we constructed a novel NIR ratiometric fluorescent probe based on the "aldehyde group auxiliary strategy", Cy-H2S, for the quantitative detection and precise imaging of H2S in living cells and zebrafish. Cy-H2S responded quickly (150 s) and was highly sensitive (0.179 μM) to H2S donor. Cy-H2S was further successfully employed to track endogenous H2S fluctuation in HCT116 cells and zebrafish and evaluated the release efficiency of the H2S prodrug in a NIR ratiometric imaging way. Cy-H2S has the potential to be used as a reliable indication of H2S levels in living cells and zebrafish, as well as an innovative and practical instrument for furthering the physiological research of H2S, which will encourage the creation of advanced NIR ratiometric probes for a variety of biological applications.