Enhancement of Activated Carbon on Anaerobic Fermentation of Heavy-Metal-Contaminated Plants: Insights into Microbial Responses

Anaerobic fermentation is a potentially cost-effective approach to disposing of metal-contaminated biowaste collected during phytoremediation. However, the compound heavy metals contained in the biowaste may limit the efficiency of anaerobic fermentation. In this study, anaerobic fermentation with alfalfa harvested from an iron tailing as the feedstock was set up and further enhanced by granular activated carbon (AC). The results showed that adding AC improved the cumulative biogas yields of alfalfa contaminated with metals (AM) by 2.26 times. At the biogas peak stage, plenty of microbes were observed on the surface of the AC, and the functional groups of AC contributed to better electron transfer, lower heavy metal toxicity and higher CH