椎间盘
软骨发生
间充质干细胞
细胞生物学
祖细胞
干细胞
克隆形成试验
环空(植物学)
生物
病理
解剖
细胞
医学
遗传学
植物
作者
Paola De Luca,Mauro Castagnetta,Laura de Girolamo,Silvia Coco,Michela Malacarne,Enrico Ragni,Marco Viganò,Gaia Lugano,Marco Brayda-Bruno,Domenico Coviello,Alessandra Colombini
摘要
Degenerative processes of the intervertebral disc (IVD) and cartilaginous endplate lead to chronic spine pathologies.Several studies speculated on the intrinsic regenerative capacity of degenerated IVD related to the presence of local mesenchymal progenitors.However, a complete characterisation of the resident IVD cell populations, particularly that isolated from the endplate, is lacking.The purpose of the present study was to characterise the gene expression profiles of human nucleus pulposus (NPCs), annulus fibrosus (AFCs) and endplate (EPCs) cells, setting the basis for future studies aimed at identifying the most promising cells for regenerative purposes.Cells isolated from NP, AF and EP were analysed after in vitro expansion for their stemness ability, immunophenotype and gene profiles by large-scale microarray analysis.The three cell populations shared a similar clonogenic, adipogenic and osteogenic potential, as well as an immunophenotype with a pattern resembling that of mesenchymal stem cells.NPCs maintained the greatest chondrogenic potential and shared with EPCs the loss of proliferation capability during expansion.The largest number of selectively highly expressed stemness, chondrogenic/tissue-specific and surface genes was found in AFCs, thus representing the most promising source of tissue-specific expanded cells for the treatment of IVD degeneration.
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