凝聚
化学
小泡
酶
脂泡
生物物理学
色谱法
生物化学
膜
生物
作者
Celina Love,Jan Steinkühler,David T. Gonzales,Naresh Yandrapalli,Tom Robinson,Rumiana Dimova,T-Y Dora Tang
标识
DOI:10.1002/ange.201914893
摘要
Abstract In situ, reversible coacervate formation within lipid vesicles represents a key step in the development of responsive synthetic cellular models. Herein, we exploit the pH responsiveness of a polycation above and below its pK a , to drive liquid–liquid phase separation, to form single coacervate droplets within lipid vesicles. The process is completely reversible as coacervate droplets can be disassembled by increasing the pH above the pK a . We further show that pH‐triggered coacervation in the presence of low concentrations of enzymes activates dormant enzyme reactions by increasing the local concentration within the coacervate droplets and changing the local environment around the enzyme. In conclusion, this work establishes a tunable, pH responsive, enzymatically active multi‐compartment synthetic cell. The system is readily transferred into microfluidics, making it a robust model for addressing general questions in biology, such as the role of phase separation and its effect on enzymatic reactions using a bottom‐up synthetic biology approach.
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