心肌炎
生物
趋化因子
炎症
信号转导
免疫学
医学
细胞生物学
内科学
作者
Tian Hao Zhao,Yi Xuan Jiang,Kai Qin Chen,Dan Qiu,Yanzhe Xu,Chun Yuan,Tao Ren,Bo Zhang,Bin Dai,Jue Hu,Jun Lü,Fang Liang Zhou,Rong Xiao,Fang guo Lu,Ke Wei
出处
期刊:Genes & Genomics
[Springer Nature]
日期:2022-06-06
卷期号:44 (12): 1593-1605
被引量:1
标识
DOI:10.1007/s13258-022-01267-4
摘要
Myocarditis is a myocardial injury that can easily cause adolescent death. Traditional research models of animal invasion with viral components, lipopolysaccharide (LPS) or porcine myocardial myosin, among others, have the shortcomings of potential biological safety hazards and high animal mortality.To explore the construction of a novel myocarditis model with cyclosporine A and the potential genes and pathways associated with it.BALB/c mice were used in this study, and cyclosporin A and LPS were injected into the peritoneal cavity of mice. The successful establishment of the model was assessed by detecting serum myocardial injury markers and inflammatory factors levels, HE, IHC staining, and RT-qPCR methods. Key genes were obtained using the GSE35182 dataset from the GEO database and validated with the RT-qPCR method.We found that a large number of inflammatory cells infiltrated the myocardium of mice in each group of Cyclosporin A constructed model, while the expression of inflammatory factor indicators was increased, and this model has the characteristics of high degree of local inflammation in myocardial tissue, low mortality, and safe and non-toxic treatment. Using GSE35182 data, we selected 18 Hub genes and validated Hub genes in myocardial tissue with RT-qPCR and found that multiple signaling pathways such as Toll-likereceptor signaling pathway(TLRs), Rap1 signal pathway(Rap1), and Chemokine signaling pathway may be involved in the development of myocarditis.Cyclosporin A can construct a new myocarditis model, and TLRs, Chemokines and Rap1 signaling pathways may be the core pathways of myocarditis.
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