Antioxidant mechanism of a newly found phenolic compound from adlay (NDPS) in HepG2 cells via Nrf2 signalling

An in-depth understanding of the bioactive mechanism of phytochemicals has a good guiding value for the design of related functional foods. Herein, the effect of N1, N5- di-[(E)-p-coumaroyl]-spermidine (NDPS) originated from adlay on protecting HepG2 cells from oxidative stress was evaluated by MTT assay, western blot and qRT-PCR. After pre-treatment of NDPS, the activities of antioxidant enzymes (including superoxide dismutase, glutathione peroxidase, γ-glutamyl cysteine synthetase and heme oxygenase-1) were increased, as well as the level of proteins and gene expressions were elevated. Moreover, the γ-GCS, HO-1, SOD and GPx protein level were enhanced for the cells with NDPS treatment compared to both positive control and negative control groups. These findings suggested that NDPS could protect HepG2 cells from oxidative stress by increasing the antioxidant enzymes regulated by Nrf2/ARE pathway.