Study on Rapid Test Method for Malachite Green,Crystal Violet and Their Metabolites Residue in Aquatic Product

[Objective] A rapid test strip for field test of malachite green(MG),crystal violet(CV) and their metabolites residue in aquatic product was established.[Method] Based on colloidal gold immnochromatographic assay,the sample pads were treated with different reactive mode,then the treated pads were used in the experiment to develop rapid field test strip of malachite green,crystal violet and their metabolites.[Result] The detection limits for MG,leucomalachite green(LMG),CV and leucocrystal violet(LCV) were both 2 μg/L,and the testing time for one sample was about 10 min.[Conclusion] As a rapid,convenient and sensitive detection method,the experimental results can be fully understood at a glance,so it can be taken as an effective screening tool for detecting malachite green,crystal violet and their metabolites residue in aquatic product.