Prolongation of skin allograft survival in mice by syngenic hematopoietic stem cell transplantation

To investigate the mechanism of tolerance of organ transplantation following syngenic hematopoietic stem cell transplantation(HSCT),the mouse model was constructed by allogenic skin transplantation,and FK506 was injected into peritoneal cavity 2 weeks later.Three weeks later,the treated group was exposed to total body irradiation and accepted the syngenic bone marrow transplantation from GFP C57BL/6 transgenic mice.The viability of chimeric mice and graft were observed constantly.The GFP expression in peripheral blood was analyzed by FACS;the specification of induced tolerance was detected by MLR.It was found that the survival time of mouse from treated group was(29.14±4.92) d,significantly longer than the control group(P0.05).The expression of GFP following BMT was 82%(4w) and 91%(6w) respectively.The outcome of MLR and DTH for the treated group had significant difference to the control,P0.05.It is concluded that syngenic HSCT combined with immunosuppressive treatment may induce tolerance to skin transplantation.