甲基化
分子生物学
GSTP1公司
复式(建筑)
DNA甲基化
化学
寡核苷酸
实时聚合酶链反应
DNA
生物
色谱法
生物化学
基因
基因表达
基因型
出处
期刊:Methods in molecular biology
日期:2009-01-01
卷期号:: 339-346
被引量:12
标识
DOI:10.1007/978-1-59745-522-0_24
摘要
The HeavyMethyl (HM) assay is a real-time PCR assay suitable for the qualitative and quantitative DNA methylation analysis of fresh, frozen, or formalin-fixed paraffin-embedded tissues and remote samples, such as serum, plasma, and urine. The HM uses a methylation-specific oligonucleotide blocker and a methylation-specific probe to achieve methylation-specific amplification and detection. A protocol for a duplex real-time PCR for the analysis of the methylation status of the GSTP1 exon1 in prostate tissue samples is presented. This chapter describes the preparation and analysis of a combined HM GSTP1 Exon1 and GSTP1 reference assay.
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