夏枯草中熊果酸的提取分离及含量测定

Objective: To study the extract technology of Ursolic Acid from Spica Prunellae,and to build the method of determining its content. Methods: Adopting the method of ethanol extract and agglutination separate to isolate Ursolic Acid.The content of Ursolic Acid in sample is determined by HPLC with Methanol-H 2 O-Glacial acetic acid-Triethylamine (83:17:0.04:0.02) as mobile phase at a flow rate of 1.0ml/min,and detection wavelength at 210nm. Beckman ODS 150mm×4.6mm column is used at 25℃. Results: The product is recognized to be Ursolic Acid by physicochemical contents and thin layer chromatography.Adopting peak area normalization method for counting.The extraction rate is 99.8%. Conclusion: The technology of isolating and preparation Ursolic Acid is advanced、pratical、reasonable and feasible. It can be applied in industrial production. The purity of Ursolic Acid is high and the safety is good. The analytical method is simple and accurate.It can be adapted to evaluate the quality of Ursolic Acid.