The Confinement of Multiple Interfaces for Self‐Enhanced Photoelectrochemical and Electrochemiluminescence Dual‐Mode Biosensor

nanocubes realized the simultaneous quench of PEC and ECL signals. Impressively, nicking endonuclease (NEase)-based DNA wheel nanostructures were quite beneficial to the low background interference and the efficient dynamic signal amplification. As a result, the proposed dual-mode biosensor achieved acetamiprid detection in a range of 1 fM to 1 nM with detection limit of 0.31 fM (PEC detection) and 0.42 fM (ECL detection). Moreover, satisfactory results were also obtained in the analysis of real samples with remarkable selectivity, stability and reproducibility, establishing the biosensor as a dependable approach for precise pesticides detection.