刀豆蛋白A
葡聚糖
鼠李糖
化学
脾细胞
多糖
甘露糖
脂多糖
肿瘤坏死因子α
半乳糖
脾脏
阿拉伯糖
免疫系统
药理学
生物化学
内分泌学
木糖
免疫学
生物
酶
发酵
体外
作者
Xianhong Xie,Jianwu Wang,Hanping Zhang
标识
DOI:10.1016/j.carbpol.2015.04.037
摘要
A water-soluble polysaccharide named as AMP was isolated and purified from the leaves of Ampelopsis megalophylla by DEAE-52 Cellulose and Sephadex G-100 column chromatography. AMP had an average molecular weight of about 8.4 × 104 Da and was composed of galactose (Gal), mannose (Man), glucose (Glc), arabinose (Ara), and rhamnose (Rha) in a molar ratio of 2.7:1.6:1.1:0.6:0.3. After 10 days of AMP (50, 100, and 200 mg/kg) treatment once daily in tumor-bearing mice, AMP oral administration could inhibit the growth of transplantable Sarcoma 180 (S180) tumor in mice and increase the spleen index and body weight. Furthermore, AMP also promote splenocytes’ proliferation induced by concanavalin A (ConA) and lipopolysaccharide (LPS), strengthen peritoneal macrophages to devour neutral red and increase the production of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in serum. These results suggest that AMP had clear antitumor activity, which might be related to its regulation of immune function in mice.
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