西塔
锌指
生物
抄写(语言学)
MHC I级
川东北74
发起人
MHC II级
基因
遗传学
转录因子
主要组织相容性复合体
分子生物学
细胞生物学
基因表达
哲学
语言学
作者
Wafa Y. Al-Kandari,Srikarthika Jambunathan,Vandana Navalgund,Rupa Koneni,Margot Freer,Neeta Parimi,Rajini Mudhasani,Joseph D. Fontes
标识
DOI:10.1016/j.molimm.2006.02.029
摘要
The class II trans-activator (CIITA) is recognized as the master regulator of major histocompatibility complex (MHC) class II gene transcription and contributes to the transcription of MHC class I genes. To better understand the function of CIITA, we performed yeast two-hybrid with the C-terminal 807 amino acids of CIITA, and cloned a novel human cDNA named zinc finger, X-linked, duplicated family member C (ZXDC). The 858 amino acid ZXDC protein contains 10 zinc fingers and a transcriptional activation domain, and was found to interact with the region of CIITA containing leucine-rich repeats. Over-expression of ZXDC in human cell lines resulted in super-activation of MHC class I and class II promoters by CIITA. Conversely, silencing of ZXDC expression reduced the ability of CIITA to activate transcription of MHC class II genes. Given the specific interaction between the ZXDC and CIITA proteins, as well as the effect of ZXDC on MHC gene transcription, it appears that ZXDC is an important regulator of both MHC class I and class II transcription.
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