17 beta-Estradiol Induced Prostatitis in the Rat is an Autoimmune Disease

No AccessJournal of UrologyInvestigative Urology1 Nov 199617 beta-Estradiol Induced Prostatitis in the Rat is an Autoimmune Disease L. Seethalakshmi, R.S. Bala, R.K. Malhotra, T. Austin-Ritchie, C. Miller-Graziano, M. Menon, and J. Luber-Narod L. SeethalakshmiL. Seethalakshmi , R.S. BalaR.S. Bala , R.K. MalhotraR.K. Malhotra , T. Austin-RitchieT. Austin-Ritchie , C. Miller-GrazianoC. Miller-Graziano , M. MenonM. Menon , and J. Luber-NarodJ. Luber-Narod View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65548-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Studies suggest that alteration in steroid hormone levels may be one of the factors causing nonbacterial prostatitis (NBP) in rats. We hypothesized that hormonally induced prostatitis in the rat may be an autoimmune disease. Studies were carried out to prove this hypothesis. Materials and Methods: We injected 17 beta-estradiol (E2; 250 microgram/kg. subcutaneously) or vehicle into 1-year-old male rats for 30 days, and isolated and cultured the splenocytes in the presence of con-A (Experiment 1). Approximately 107 splenocytes were adoptively transferred into young naive syngeneic rats. To find out whether or not the inflammation is mediated by T-lymphocytes, a pure population of T-lymphocytes from E2 -treated 3-month-old rats was injected into young naive syngeneic rats (Experiment 2). To rule out the possibility that the inflammation was due to con-A itself, we cross-linked some T-cells with anti-CD3 antibody before adoptive transfer (Experiment 2). Results: The recipients of splenocytes from E2 -treated animals presented evidence of inflammation in terms of lymphocytic infiltration and presence of degranulated mast cells. Furthermore, we observed in these animals an increase in histamine-releasing peptide (HRP) levels, an indication of plasma extravasation. The T-cells stimulated by anti-CD3 antibody produced a similar degree of inflammation, thereby ruling out the possibility that the inflammation was due to con-A. The results also indicated that the immune response to antigen(s) is not dependent on the age of the animal but is dependent on a population of CD3 sup + T-cells. Conclusion: Our results demonstrate that hormonal imbalance and autoimmunity in male rats produce the symptoms of nonbacterial prostatitis. References 1 : Prostatitis and related disorders. In: Cambell's Urology. Edited by . Philadelphia: W. B. Saunders Co.1986. Google Scholar 2 : The role of androgens and estrogens in the pathogenesis of experimental nonbacterial prostatitis. J. Urol.1988; 140: 1049. 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Abstract, Google Scholar Divisions of Urologic Transplantation and Surgery, and Research, Department of Surgery, and the Department of Pathology, University of Massachusetts Medical Center, Worcester, Massachusetts, and the Department of Pathology, Little Company of Mary Hospital, Chicago, Illinois.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byDone J, Rudick C, Quick M, Schaeffer A and Thumbikat P (2018) Role of Mast Cells in Male Chronic Pelvic PainJournal of Urology, VOL. 187, NO. 4, (1473-1482), Online publication date: 1-Apr-2012. Volume 156Issue 5November 1996Page: 1838-1842 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information L. Seethalakshmi More articles by this author R.S. Bala More articles by this author R.K. Malhotra More articles by this author T. Austin-Ritchie More articles by this author C. Miller-Graziano More articles by this author M. 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