细胞毒性
PLGA公司
体内
MTT法
PEG比率
多西紫杉醇
分散性
纳米颗粒
化学
材料科学
体外
粒径
药理学
核化学
纳米技术
癌症
医学
生物化学
有机化学
生物技术
财务
物理化学
经济
内科学
生物
作者
Mona Noori Koopaei,Mohammad Reza Khoshayand,Seyed Hossein Mostafavi,Mohsen Amini,Mohammad Reza Khorramizadeh,Mahmood Jeddi Tehrani,Fatemeh Atyabi,Rassoul Dinarvand
出处
期刊:PubMed
日期:2014-01-01
被引量:32
摘要
In this study a 3-factor, 3-level Box-Behnken design was used to prepare optimized docetaxel (DTX) loaded pegylated poly lactide-co-glycolide (PEG-PLGA) Nanoparticles (NPs) with polymer concentration (X1), drug concentration (X2) and ratio of the organic to aqueous solvent (X3) as the independent variables and particle size (Y1), poly dispersity index (PDI) (Y2) and drug loading (Y3) as the responses. The cytotoxicity of optimized DTX loaded PEG-PLGA NPs was studied in SKOV3 tumor cell lines by standard MTT assay. The in-vivo antitumor efficacy of DTX loaded PLGA-PEG NPs was assessed in tumor bearing female BALB/c mice. The optimum level of Y1, Y2 and Y3 predicted by the model were 188 nm, 0.16 and 9% respectively with perfect agreement with the experimental data. The in-vitro release profile of optimum formulation showed a burst release of approximately 20% (w/w) followed by a sustained release profile of the loaded drug over 288 h. The DTX loaded optimized nanoparticles showed a greater cytotoxicity against SKOV3 cancer cells than free DTX. Enhanced tumor-suppression effects were achieved with DTX-loaded PEG-PLGA NPs. These results demonstrated that optimized NPs could be a potentially useful delivery system for DTX as an anticancer agent.
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