抑制因子
生物
trp操纵子
操作员(生物学)
大肠杆菌
色氨酸
DNA
脚印
结合位点
甲基化
突变体
操纵子
细胞生物学
遗传学
分子生物学
基因
转录因子
氨基酸
基序列
作者
A A Kumamoto,William G. Miller,Robert P. Gunsalus
出处
期刊:Genes & Development
[Cold Spring Harbor Laboratory]
日期:1987-08-01
卷期号:1 (6): 556-564
被引量:93
摘要
DNase I footprinting and methylation protection studies have been used to analyze the binding of Escherichia coli Trp repressor to the trpR, aroH, and trp operators. The methylation protection assay shows that Trp repressor binds in two successive major grooves of the trpR operator, three successive major grooves of the aroH operator, and four successive major grooves of the trp operator. The simplest model that explains the difference in Trp repressor interaction at the three operators is that the aroH and trp operators are composed of multiple, helically stacked binding sites. When viewed in three dimensions, each site is positioned on a different face of the DNA, and together process up the surface of the DNA helix. Analysis of a deletion derivative of the trp operator supports this model.
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