High-performance liquid chromatographic elution behaviour of oligosaccharides, monosaccharides and sugar degradation products on series-connected ion-exchange resin columns using water as the mobile phase

The high-performance liquid chromatographic separation of oligosaccharides, monosaccharides, sugar degradation products and alcohols using a series-connected system of different ion-exchange columns with water as the eluent was investigated. The non-additivity of the number of theoretical plates (N), the height equivalent to a theoretical plate (H) and the resolution (RS) on ion-exchange phases is discussed.