A NovelL-Amino Acid Ligase Is Encoded by a Gene in the Phaseolotoxin Biosynthetic Gene Cluster fromPseudomonas syringaepv.phaseolicola1448A

In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPPH_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various hetero-dipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.