Effect and mechanism research of solanine on prostate cancer cell LNCaP and Du145

Objective To investigate the role of solanine in human androgen-dependent prostate cancer cell line LNCaP and androgen-independent prostate cancer cell Du145.Methods The influence on the proliferation of LNCaP and Du145 cells were evaluated by MTT assay.Cell cycle and cell apoptosis of LNCaP and Du145 were assessed by flow cytometry and fluorescence microscope.The protein of expressions of Bcl-2,Bax,and Caspase-3 were determined by Western blot.Results Solanine could significantly inhibit the proliferation of the two cells in time-and dose-dependent manners(P0.05);also induced S-phase arrest of LNCaP and Du145 cells.The apoptosis ratio in treatment groups were significantly higher than that in 0 μg/mL group,with early apoptosis.Significant down-regulation of Bcl-2 and Caspase-3 was induced by solanine,with no effect on Bax protein.Conclusion Solanine provides anti-prostate cancer effect by inhibiting cell proliferation,S-phase arresting,and inducing apoptosis.