[Time-effect and dose-effect relationships study on the effect of tonifying kidney and activating blood formulas contained serum on MSCs proliferation and osteogenic differentiation].

To screen the optimal cell proliferation and osteogenic differentiation concentration of SD rats' Mesenchymal stem cells (MSCs) affected by compound Bushenhuoxue (CB) contained serum.30 SD female rats, aged 10 months, were randomly divided into low-dose (11.6 g/kg), medium dose (34.8 g/kg) and high dose CB group (104.4 g/kg), 10 rats in each group (qd, 12 d). Rats blood of three groups were taken respectively in 0.5, 1.0, 1.5, 2.0, 3.0 h after the last intragastric infusion, blood serum was separated, and different concentrations of CB contained serum were given to MSCs form SD female rats aged 3 months. MTT method was used to determine the effect of CB on MSCs proliferation. Alkaline phosphatase (ALP) was detected to determine the osteogenic differentiation of MSCs from each group.The 25% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with low dose CB was proved to be the most effective concentration on the proliferation of MSCs; The 25%-30% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with high dose CB was proved to be the most effective concentration on the differentiations of MSCs into osteoblast.CB contained serum could promote the proliferation and osteogenic differentiation of MSCs.